P8742
Sigma
Posttranslational Modification of Proteins: Tools for Functional Proteomics
| ISBN-10: | 0-89603-678-2 |
| ISBN-13: | 978-0-89603-678-9 |
Description
| General description | This volume describes reproducible methods for detecting and analyzing the posttranslational modifications of protein, particularly with regard to protein function, proteome research, and the characterization of pharmaceutical proteins. Methods include those for analyzing the assignment of disulfide bond sites in proteins, protein N-glycosylation and protein O-glycosylation, and oligosaccharides present at specific single glycosylation sites in a protein. Additional techniques facilitate the analysis of glycosylphosphatidylinositols, lipid modifications, protein phosphorylation and sulfation, protein methylation and acetylation, a-amidation and lysine hydroxylation |
Properties
| publication info | C. Kannicht, Humana Press, 2002, 385 pp., hard cover |
Table Of Contents
| Table of Contents | Assignment of Disulfide Bonds in Proteins by Chemical Cleavage and Peptide Mapping by Mass Spectrometry Carbohydrate Composition Analysis of Glycoproteins Using Highly Sensitive Fluorescence Detection Methods Enzymatical Hydrolysis of N-Glycans from Glycoproteins and Fluorescent Labeling by 2-Aminobenzamide (2-AB) Separation of N-Glycans by HPLC, Enzymatic Sequence Analysis of N-Glycans Immunological Detection of O-GlcNAc Analysis of O-Glycosylation Characterization of Site-Specific Glycosylation Monitoring Glycosylation of Therapeutic Glycoproteins for Consistency Using Highly Fluorescent Anthranilic Acid Metabolic Labeling and Structural Analysis of Glycosylphosphatidylinositols from Parasitic Protozoa Analysis of S-Acylation of Proteins Immunoblotting Methods for the Study of Protein Ubiquitination Analysis of Methylation and Acetylation in E. coli Ribosomal Proteins Identification of In Vivo Protein Phosphorylation Sites with Mass Spectrometry Analysis of Tyrosine-O-Sulfation a-Amidated Peptides: Approaches for Analysis g-Glutamate and b-Hydroxyaspartate in Proteins Detection of isoAspartate Residues as a Posttranslational Modification of Proteins and Peptides Lysine Hydroxylation and Crosslinking of Collagen Heterologous Expression in Endocrine Cells for Analysis of Posttranslational Modifications 2D-Electrophoresis: Detection of Glycosylation and Influence on Spot Pattern |
